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The dataset was analysed with the following parameters: COBRA Toolbox v3 [14], Escher [65], and RAVEN [25]. The parameter settings in RAVEN v2.1.3 are described in Additional file 2: Table S3. Detailed descriptions of the metabolic models, including the model conditions, metabolic reactions, and the nutrients and metabolites are listed in Additional file 1: Table S4. The script to reconstruct a metabolic model is provided in Additional file 1: Table S5.
In order to validate the generated model, a biomass reaction was firstly generated for the model to be tested. This was achieved by importing a model from Escher online and then exporting the biomass reaction as a MAT-file to be used as a template for subsequent computations. The biomass reaction of the model was calculated using the COBRA metabolic model analysis toolbox and imported into Escher online to be visualised in Escher online (Additional file 2: Figure S4). A sample of the biomass reaction is shown in Additional file 2: Figure S4
[3] G. J. L. Konijnendijk, J. H. Hubach, C. M. Lehner, P. C. B. Edwards, and J. K. Segersvärd, “The Hox cluster of the marine gastropod Littorina littorea: Identification of a partially duplicated, tandemly arrayed cluster,” Comparative Genomics 11.6 (2010) doi: 10.1186/1471-2164-11-589
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