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To determine the location and developmental regulation of transforming growth factor-beta (TGF-beta) and its functional receptor during lens development. Northern blot analysis was used to determine the expression of TGF-beta1, TGF-beta2, and TGF-beta type II receptor (TbetaRII) in mouse lens at different developmental stages. The specificity of each antibody was verified by Western blot analysis using recombinant TGF-beta1. In situ hybridization was performed to determine the expression pattern of TGF-beta1, TGF-beta2, and TbetaRII in lens. Immunohistochemistry was used to localize the distribution of TGF-beta1, TGF-beta2, and TbetaRII in the lens. Transforming growth factor-beta1 and TGF-beta2 were expressed in mouse lens during development. TbetaRII was also detected by Western blot analysis. The distributions of TGF-beta1 and TGF-beta2 and the expression of TbetaRII were correlated with that of crystallins. Both TGF-beta1 and TGF-beta2 were localized to the posterior lens epithelium. Transforming growth factor-beta1 was detected at day 15 of gestation (E15) and was present in the nucleus at E16. TGF-beta2 was first detected at E15 and later in the nucleus and cytoplasm. Transforming growth factor-beta1 and TGF-beta2 colocalized with crystallins at E16 and thereafter. Transforming growth factor-beta1 was detected throughout the lens at day E19, which then decreased 0b46394aab
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